Cellular responses to photodynamic therapy

Kristian Berg; Qian Peng; Jahn M. Nesland; Johan Moan

doi:10.1117/12.168664

1 March 1994 Cellular responses to photodynamic therapy

Kristian Berg, Qian Peng, Jahn M. Nesland, Johan Moan

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168664
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The mechanisms of photoinactivation of NHIK 3025 cells in culture sensitized by sulfonated tetraphenyl porphines (TPPS_n) are described. Di- and tetrasulfonated species are mainly located in lysosomes. TPPS₁ is located diffusely in the extranuclear space, supposedly bound to endoplasmic reticulum as indicated by electron microscopical findings, and to some extent in lysosomes. After PDT TPPS_n penetrates the lysosomal membrane. However, after exposure of cells to TPPS₄ and light lysosomal enzymes are inactivated before they eventually can be released to the cytosol. In all cases electron microscopical studies show swollen secondary lysosomes after PDT. TPPS_n and light induce accumulation of cells in mitosis. This is due to photochemical damage to the unpolymerized form of tubulin.

Citation Download Citation

Kristian Berg, Qian Peng, Jahn M. Nesland, and Johan Moan "Cellular responses to photodynamic therapy", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168664

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