In vitro photosensitizing activity of benzoporphyrin derivative monoacid ring A using pulsed and continuous-wave irradiation

Heyke Cacile Diddens; Robert Gillies; Tayyaba Hasan

doi:10.1117/12.168655

1 March 1994 In vitro photosensitizing activity of benzoporphyrin derivative monoacid ring A using pulsed and continuous-wave irradiation

Heyke Cacile Diddens, Robert Gillies, Tayyaba Hasan

Author Affiliations +

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168655
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Benzoporphyrin derivative monoacid ring A (BPD-MA) is a potent new photosensitizer with a significant extinction coefficient at 690 nm. In this study, the photosensitizing efficacy of BPD-MA irradiated at 694 nm with continuous wave (cw) radiation from an argon ion laser pumped dye laser and high intensity pulsed radiation from a ruby laser was compared. Human bladder carcinoma cells in vitro were treated with 0.14 (mu) M BPD-MA for 3 h, washed, then irradiated with either cw or pulsed irradiation. A cumulative cw radiant exposure of 1 or 3 J/cm² led to cellular survival rates of 38% and 6% respectively compared to untreated controls. Essentially no phototoxicity was observed at the high intensity pulsed powers used in this study.

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Heyke Cacile Diddens, Robert Gillies, and Tayyaba Hasan "In vitro photosensitizing activity of benzoporphyrin derivative monoacid ring A using pulsed and continuous-wave irradiation", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168655

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