Induction of protoporphyrin biosynthesis and photodynamic inactivation of B16 melanoma cells

Zvi Malik; T. Babushkina; M. Shafran; Y. Nordenberg; R. Mamet; N. Schoenfeld

doi:10.1117/12.168674

1 March 1994 Induction of protoporphyrin biosynthesis and photodynamic inactivation of B16 melanoma cells

Zvi Malik, T. Babushkina, M. Shafran, Y. Nordenberg, R. Mamet, N. Schoenfeld

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168674
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The induction of protoporphyrin (PP) synthesis in B16 melanoma cells and their subsequent photodestruction was investigated. Photosensitized cells gradually disintegrated following prolonged irradiation. The photosensitized cells showed an immediate potassium loss compensated only partially by influx of Na and other ions. The dominant characteristics of these cells were potassium efflux, water influx and ultrastructural damage in subcellular compartments. In conclusion, a highly efficient system for killing B16 melanoma cells was achieved by photosensitization of endogenous PP produced subsequent to chemical induction.

Citation Download Citation

Zvi Malik, T. Babushkina, M. Shafran, Y. Nordenberg, R. Mamet, and N. Schoenfeld "Induction of protoporphyrin biosynthesis and photodynamic inactivation of B16 melanoma cells", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168674

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available