Kinetics of the photodynamic damage of phthalocyanine-loaded cultured K562 cells studied by means of flow cytometry

Elena B. Chernyaeva; Jan Greve; Bart G. de Grooth

doi:10.1117/12.168681

1 March 1994 Kinetics of the photodynamic damage of phthalocyanine-loaded cultured K562 cells studied by means of flow cytometry

Elena B. Chernyaeva, Jan Greve, Bart G. de Grooth

Author Affiliations +

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168681
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The kinetics of cellular damage after photodynamic action was studied by means of flow- cytometry. Cultured K562 cells were loaded with tetrasulphonated aluminum phthalocyanine and propidium iodide, acridine orange and rhodamine 123 were used to assess the damage done to the cells. Exposure of phthalocyanine-loaded cells to light resulted in the following order of events: increase of the intracellular Pc fluorescence -> mitochondrial damage -> changes of the cell surface and/or volume -> plasma membrane potential vanishing -> severe damage of cell plasma membrane. For all these processes it takes approximately two hours to reach the stationary state.

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Elena B. Chernyaeva, Jan Greve, and Bart G. de Grooth "Kinetics of the photodynamic damage of phthalocyanine-loaded cultured K562 cells studied by means of flow cytometry", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168681

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