Merocyanine 540 and Photofrin II as photosensitizers for in vitro killing of duck hepatitis B virus and human hepatoma cells

Tsung-I. Lin; Yong-Shau Shien; Ming-Chien Kao

doi:10.1117/12.168669

1 March 1994 Merocyanine 540 and Photofrin II as photosensitizers for in vitro killing of duck hepatitis B virus and human hepatoma cells

Tsung-I. Lin, Yong-Shau Shien, Ming-Chien Kao

Author Affiliations +

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168669
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The feasibility of using merocyanine 540 (MC 540) and Photofrin II (PII) as effective photodynamic therapeutic (PDT) agents for killing hepatoma cells and duck hepatitis B virus (DHBV) in vitro was investigated. Cultured duck hepatocytes infected with DHBV and hepatoma cells, Hep 3B and HCC 36, were used as models. MC 540 and PII effectively inhibits the DHBV growth by 90 - 99% in a dose- and light-dependent manner. Photodynamic killing of MC 540 in the two hepatoma cell lines results in 94 - 99% growth inhibition. However, both photosensitizers exhibit dark cytotoxicity (37 - 56%). The present results suggest that MC 540 and PII could be promising and effective photodynamic agents for killing HBV and hepatoma cells.

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Tsung-I. Lin, Yong-Shau Shien, and Ming-Chien Kao "Merocyanine 540 and Photofrin II as photosensitizers for in vitro killing of duck hepatitis B virus and human hepatoma cells", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168669

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