Time-gated fluorescence imaging of different organs in tumor-bearing mice after porphyrin administration

Rinaldo Cubeddu; Gianfranco L. Canti; Mario Musolino; Antonio Pifferi; Paola Taroni; Gianluca Valentini

doi:10.1117/12.166813

15 January 1994 Time-gated fluorescence imaging of different organs in tumor-bearing mice after porphyrin administration

Rinaldo Cubeddu, Gianfranco L. Canti, Mario Musolino, Antonio Pifferi, Paola Taroni, Gianluca Valentini

Author Affiliations +

Proceedings Volume 2081, Optical Biopsy; (1994) https://doi.org/10.1117/12.166813
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

A time-gated fluorescence imaging technique was applied on tumor-bearing porphyrin-treated mice to study the sensitizer distribution in different organs and tissue types, and to establish whether false positives in the diagnosis of tumors (based on porphyrin fluorescence) could be generated by this localization in healthy tissues. Mice were administered 25 mg/kg body weight (b.w.) of HpD or 5 mg/kg b.w. of PII, and sacrificed 8 hr later. Time- gated fluorescence images were acquired from tumor, skin, muscle, fat, brain, heart, lung, lymph nodes, liver, bowel, spleen, and bone of both treated and untreated animals. Similar results were obtained with HpD and PII. The presence of porphyrins clearly helps the localization of the neoplastic area, which is characterized by the strongest fluorescence in delayed images. An appreciable long-living emission was observed also in bones. With the exception of the bowel, the fluorescence of other organs was weaker and, in untreated mice, short-living.

Citation Download Citation

Rinaldo Cubeddu, Gianfranco L. Canti, Mario Musolino, Antonio Pifferi, Paola Taroni, and Gianluca Valentini "Time-gated fluorescence imaging of different organs in tumor-bearing mice after porphyrin administration", Proc. SPIE 2081, Optical Biopsy, (15 January 1994); https://doi.org/10.1117/12.166813

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