Intracellular phthalocyanine localization: confocal laser scanning microscopy studies

Elena B. Chernyaeva; Jan Greve; Bart G. de Grooth; A. G.J.M. Van Leeuwen

doi:10.1117/12.167421

1 February 1994 Intracellular phthalocyanine localization: confocal laser scanning microscopy studies

Elena B. Chernyaeva, Jan Greve, Bart G. de Grooth, A. G.J.M. Van Leeuwen

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167421
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Phthalocyanines (Pc) are promising second-generation photosensitizers for the photodynamic therapy (PDT) of cancer. We report on the tetrasulfonated aluminum phthalocyanine (AlPcS₄) localization in cultured Chinese hamster lung cells studied by means of confocal laser scanning microscopy (CLSM). In these cells AlPcS₄ was found in granules surrounding Golgi apparatus and in the peripheral cytoplasmic region. Peripheral Pc-containing granules partially coincided with the acidic cellular compartments. The effect of irradiation with light on Pc intracellular distribution was also studied. In the Pc-free medium disruption of some Pc- containing granules was observed followed by appearance of Pc fluorescence in the cell plasma membrane, the nuclear envelope, and the near-nuclear region. When cells were irradiated in the presence of Pc in external medium a drastic increase of membrane permeability to Pc was observed, followed by Pc binding the cell plasma membrane, nuclear envelope, and some structures in the cytoplasm. Diffusive Pc fluorescence in the nucleus was also observed. The implication of observed Pc redistribution caused by irradiation with light for the PDT protocol is discussed.

Citation Download Citation

Elena B. Chernyaeva, Jan Greve, Bart G. de Grooth, and A. G.J.M. Van Leeuwen "Intracellular phthalocyanine localization: confocal laser scanning microscopy studies", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167421

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