Potent photosensitizers for PDT of cancer: application of confocal laser scanning microscopy for fluorescence detection of photosensitizing fluorophores in neoplastic cells and tissues

Qian Peng; Havard E. Danielsen; Johan Moan

doi:10.1117/12.167422

1 February 1994 Potent photosensitizers for photodynamic therapy of cancer: application of confocal laser scanning microscopy for fluorescence detection of photosensitizing fluorophores in neoplastic cells and tissues

Qian Peng, Havard E. Danielsen, Johan Moan

Author Affiliations +

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167422
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

By using confocal laser scanning microscopy (CLSM), intracellular and intratumoral fluorescence localization of several promising photosensitizing fluorophores, such as the derivatives of sulfonated aluminum phthalocyanines (AlPCS_n, n equals 1-4), meso- tetra(hydroxyphenyl)porphine (m-THPP) and its chlorin (m-THPC) was studied in several cancer cell lines in vitro and in tumor models in vivo. The correlation of the subcellular and intratumoral localization patterns of the potent photosensitizers with the targets of photodynamic action is discussed. Also, the advantages of using CLSM for studying the fluorescence properties of the photosensitizing fluorochromes in a biological system is emphasized.

Citation Download Citation

Qian Peng, Havard E. Danielsen, and Johan Moan "Potent photosensitizers for photodynamic therapy of cancer: application of confocal laser scanning microscopy for fluorescence detection of photosensitizing fluorophores in neoplastic cells and tissues", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167422

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