Quantitative bioluminescence imaging--a method for the detection of metabolite distributions in frozen tissues

Wolfgang Mueller-Klieser; Stefan Walenta; Georg Schwickert

doi:10.1117/12.167416

1 February 1994 Quantitative bioluminescence imaging--a method for the detection of metabolite distributions in frozen tissues

Wolfgang Mueller-Klieser, Stefan Walenta, Georg Schwickert

Author Affiliations +

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167416
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

A novel technique allows for measurement of metabolite distributions in tissue cryosections at a microscopic level using bioluminescence, single photon imaging, and computerized image analysis. Metabolites, such as ATP, glucose and lactate are registered in absolute concentration units, and the respective images can be correlated with each other and with histological structures by specific algorithms. One striking difference between malignant tumors and normal tissue is the pronounced heterogeneity of metabolite distributions in malignancies contrasted by rather homogeneous patterns obtained in many normal organs. The heterogeneous distribution of metabolites in solid tumors reflects the chaotic organization of the histological architecture and of the microvascular supply in cancerous tissue. Pixel-to-pixel comparison of metabolite distributions measured in cervix cancers of patients revealed a negative linear correlation between glucose and ATP concentrations at identical locations. In contrast, local lactate concentration was positively correlated with ATP.

Citation Download Citation

Wolfgang Mueller-Klieser, Stefan Walenta, and Georg Schwickert "Quantitative bioluminescence imaging--a method for the detection of metabolite distributions in frozen tissues", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167416

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available