Solid surface measurement of room temperature phosphorescence of Pd-coproporphyrin and its application for time-resolved microscopy

Alexander P. Savitsky; Ekaterina Yu. Mantrova; Marina V. Demcheva; Gely V. Ponomarev; Ilkka Hemmila; Erkki J. Soini

doi:10.1117/12.167419

1 February 1994 Solid surface measurement of room temperature phosphorescence of Pd-coproporphyrin and its application for time-resolved microscopy

Alexander P. Savitsky, Ekaterina Yu. Mantrova, Marina V. Demcheva, Gely V. Ponomarev, Ilkka Hemmila, Erkki J. Soini

Author Affiliations +

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167419
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

In quantitative fluorescence immunological determinations of an antibody or an antigen the measurement is usually provided with an easily detectable marker. A special problem in fluorescence determinations is that serum, microorganisms, and sections have a relatively strong autofluorescence that gives rise to high background levels for most fluorescence markers. A general approach for the elimination of background emission of biological substances is time-resolves measurement with microsecond resolution. For excitation in the UV range, especially for surface measurement, it is necessary to use special optical components with low background phosphorescence, fluorescence, afterglow, and high UV transmittance. Such optical components are not easily available. The requirement of a marker that is suitable for the surface measurement of biological substances are that in addition to the requirement for solution measurement the excitation wavelengths must be in the visible range. In this paper metalloporphyrin phosphorescent markers meeting this requirement are described.

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Alexander P. Savitsky, Ekaterina Yu. Mantrova, Marina V. Demcheva, Gely V. Ponomarev, Ilkka Hemmila, and Erkki J. Soini "Solid surface measurement of room temperature phosphorescence of Pd-coproporphyrin and its application for time-resolved microscopy", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167419

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