In situ FTIR microspectroscopy of extravasated blood-damaged brain tissue

David L. Wetzel; Steven M. Le Vine

doi:10.1117/12.166600

31 January 1994 In situ FTIR microspectroscopy of extravasated blood-damaged brain tissue

David L. Wetzel, Steven M. Le Vine

Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166600
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

Fourier transform infrared (FT-IR) microspectroscopy enables the collection of infrared spectra from microscopic regions of tissue sections. The objectives of this study were to utilize FT-IR microspectroscopy to analyze the spatial distribution of chemical changes that result from the extravasation of blood into the brain and to determine if products of free radical damage are associated with the damaged areas. An animal model that involves the injection of blood into the white matter of rat brains was used. Maps depicting the relative concentrations of chemical functional groups of lesioned sites and surrounding areas were made. Significant decreases were observed for CH₂, C equals O, P equals O, and HO-C-H functional groups at the lesioned site and penumbra regions compared to the neighboring normal tissue areas.

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David L. Wetzel and Steven M. Le Vine "In situ FTIR microspectroscopy of extravasated blood-damaged brain tissue", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166600

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