Effect of CO2 laser amputation on hydra regeneration

L. De Petrocellis; Andrea Finizio; R. Minei; Pasquale Mormile; Giovanni Pierattini

doi:10.1117/12.183129

10 August 1994 Effect of CO₂ laser amputation on hydra regeneration

L. De Petrocellis, Andrea Finizio, R. Minei, Pasquale Mormile, Giovanni Pierattini

Proceedings Volume 2097, Laser Applications; (1994) https://doi.org/10.1117/12.183129
Event: Laser Optics, 1993, St. Petersburg, Russian Federation

Abstract

In order to investigate laser effects on biological specimens, Hydra, a coelenterate with high regeneration rate, was observed for ten days during regeneration after CO₂ laser cutting. Control animals were cut with a razor blade immediately below the tentacle whorl under a dissecting microscope while they were in small glass petri dishes. They regenerated tentacles completely 8 to 10 days from the cutting. Hydra were cut in the same position with CO₂ laser. As a first step, we studied the effect of the laser beam on the normal behaviour of hydra. For the cutting, we used four different power intensities: 0.5, 1.0, 1.5 and 2.0 W. At different power intensities the animals regenerated the tentacles. However in about 20% of the animals the amputation performed with 1 and 1.5 W originated a quicker regeneration of tentacles. No effect was observed on asexual reproduction of the polyps and therefore also no change of the bud index.

Citation Download Citation

L. De Petrocellis, Andrea Finizio, R. Minei, Pasquale Mormile, and Giovanni Pierattini "Effect of CO₂ laser amputation on hydra regeneration", Proc. SPIE 2097, Laser Applications, (10 August 1994); https://doi.org/10.1117/12.183129

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