Near-field fluorescence microscopy and spectroscopy: applications to single chromophores, single proteins, and photosynthetic membranes

Sunney Xie; Errol V. Allen; Gary R. Holtom; Robert C. Dunn; Laurens Mets

doi:10.1117/12.182708

17 August 1994 Near-field fluorescence microscopy and spectroscopy: applications to single chromophores, single proteins, and photosynthetic membranes

Sunney Xie, Errol V. Allen, Gary R. Holtom, Robert C. Dunn, Laurens Mets

Author Affiliations +

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182708
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Near-field fluorescence microscopy combines nanometer resolution with the ability to conduct optical measurements with high sensitivity. To illustrate the potential of the near-field technique, we summarize recent results from our laboratory which include the fluorescence imaging of single dye molecules and single proteins and picosecond fluorescent lifetime measurements on intact photosynthetic membranes, all done at room temperature with nanometric spatial resolution.

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Sunney Xie, Errol V. Allen, Gary R. Holtom, Robert C. Dunn, and Laurens Mets "Near-field fluorescence microscopy and spectroscopy: applications to single chromophores, single proteins, and photosynthetic membranes", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182708

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