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17 August 1994 Origin of fluorescence and phosphorescence signals re-emitted from tissues
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Noninvasive, lifetime-based sensing in tissues depends upon deconvolving the probe lifetime from the photon `time-of-flights' which arise from the strong scattering properties of tissues. In this work, we investigated the utility of employing long-lived probes in order to minimize the contribution of photon migration to the decay kinetics of re-emitted, near IR light. Employing finite element techniques, we computed the spatial and temporal maps of the emission and excitation fluence for the cases of uniform and nonuniform distributions of embedded optical probe. Computations were performed for an incident impulse of light at the surface of tissues containing optical probes with varying lifetimes, (tau) . The results demonstrate that when the probe lifetime is significantly longer than the times associated with photon migration, the origin of the re-emitted light becomes located closer to the tissue surface and the `apparent' image of a fluorescent heterogeneity may be displaced from the true position.
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Eva Marie Sevick-Muraca, Lee F. Suddeath, and Christina L. Hutchinson "Origin of fluorescence and phosphorescence signals re-emitted from tissues", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994);

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