Tropomyosin rotational dynamics in thin filaments

Jun-chih Lo; Richard D. Ludescher

doi:10.1117/12.182760

17 August 1994 Tropomyosin rotational dynamics in thin filaments

Jun-chih Lo, Richard D. Ludescher

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182760
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

We have used phosphorescence anisotropy from erythrosin-5- iodoacetamide covalently attached to rabbit skeletal muscle tropomyosin (Tm) to characterize the molecular dynamics of Tm on the surface of F-actin. Although the probe was loosely bound to Tm, it was able to monitor the molecular dynamics of the F- actin/Tm complex on the microsecond time scale. The steady-state phosphorescence anisotropy of Tm in the complex was 0.025+/- 0.005 at 20 degree(s)C. Since the anisotropy of actin in a similar complex was 0.11+/- 0.01, Tm may be moving independently on the filament surface. Studies of the effect of temperature and solution viscosity provided additional evidence for independent tropomyosin motions. A modified Perrin plot of 1/r versus (tau) T/(eta) (where r is the steady-state phosphorescence anisotropy, (tau) is the phosphorescence lifetime, T is the absolute temperature, and (eta) is solution viscosity) provided evidence for two distinct modes of motion for Tm on F-actin; these modes corresponded to the rotation of segments with significantly different volumes. While the volume of the fast component was comparable to that of a single tropomyosin molecule, providing evidence that Tm moves on the surface of F- actin, the volume of the slow component corresponded to only a small portion of the entire complex.

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Jun-chih Lo and Richard D. Ludescher "Tropomyosin rotational dynamics in thin filaments", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182760

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KEYWORDS

Anisotropy

Phosphorescence

Thulium

Luminescence

Proteins

Molecules

Motion measurement

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