Comparison of analytical calculations with experimental measurements for polarized light scattering by microorganisms

Patricia G. Hull; Felecia G. Shaw; Mary S. Quinby-Hunt; Daniel B. Shapiro; Arlon J. Hunt; Terrence Leighton

doi:10.1117/12.190107

26 October 1994 Comparison of analytical calculations with experimental measurements for polarized light scattering by microorganisms

Patricia G. Hull, Felecia G. Shaw, Mary S. Quinby-Hunt, Daniel B. Shapiro, Arlon J. Hunt, Terrence Leighton

Author Affiliations +

Proceedings Volume 2258, Ocean Optics XII; (1994) https://doi.org/10.1117/12.190107
Event: Ocean Optics XII, 1994, Bergen, Norway

Abstract

The consequences of light scattering from both spherical and non-spherical particles on the propagation of light in the ocean were investigated. The scattering from an ensemble of non- spherical micro-organisms is calculated using the coupled-dipole approximation with an orientational average over Euler angles using Gauss-Legendre integration. Mie calculations provide rigorous solutions for spherical particles and are considerably less computer intensive than the coupled-dipole approximation. Furthermore, they have been shown to accurately predict the scattering for marine organisms that are nearly spherical. Scattering matrix elements calculated using the coupled-dipole approximation were compared with those obtained using Mie calculations in the limit as an ellipsoidal object approaches a sphere in order to assess the limits of applicability of the Mie theory to ellipsoidal particles. Experimental measurements of the scattering matrix elements for spherical particles (latex spheres) and ellipsoidal particles (Bacillus subtilis) were used to test the validity of our analytical approach.

Citation Download Citation

Patricia G. Hull, Felecia G. Shaw, Mary S. Quinby-Hunt, Daniel B. Shapiro, Arlon J. Hunt, and Terrence Leighton "Comparison of analytical calculations with experimental measurements for polarized light scattering by microorganisms", Proc. SPIE 2258, Ocean Optics XII, (26 October 1994); https://doi.org/10.1117/12.190107

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