Biosensor technology for the detection of illegal drugs II: antibody development and detection techniques

Reinhold Hilpert; Christian Bauer; Florian Binder; Michael Grol; Klaus Hallermayer; Hans-Peter Josel; Christian Klein; Josef Maier; Alexander Makower; Helmut Oberpriller; Josef Ritter

doi:10.1117/12.189163

6 October 1994 Biosensor technology for the detection of illegal drugs II: antibody development and detection techniques

Reinhold Hilpert, Christian Bauer, Florian Binder, Michael Grol, Klaus Hallermayer, Hans-Peter Josel, Christian Klein, Josef Maier, Alexander Makower, Helmut Oberpriller, Josef Ritter

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Proceedings Volume 2276, Cargo Inspection Technologies; (1994) https://doi.org/10.1117/12.189163
Event: SPIE's 1994 International Symposium on Optics, Imaging, and Instrumentation, 1994, San Diego, CA, United States

Abstract

In a joint project of Deutsche Aerospace, Boehringer Mannheim and the University of Potsdam portable devices for the detection of illegal drugs, based on biosensor technology, are being developed. The concept enrichment of the drug from the gas phase and detection by immunological means. This publication covers the development of specific antibodies and various detection procedures. Antibodies with a high affinity for cocaine have been developed with the aid of specially synthesized immunogens. A competitive detection procedure with biosensors based on optical grating couplers and applying particulate labels has been established, showing a lower detection limit of 10^-10 mol/l for cocaine. Additionally, a combination of a displacement-immunoreactor and an enzymatically amplified electrode was investigated, which at present still suffers from insufficient sensitivity of the immunoreactor. An alternative, fleece-matrix based test procedure, where enrichment and detection steps are integrated in a single unit, is promising in terms of simplicity and sensitivity. A simple swab-test for the detection of cocaine at surfaces has been developed, which has a lower detection limit of about 10 ng and which can be performed within one minute.

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Reinhold Hilpert, Christian Bauer, Florian Binder, Michael Grol, Klaus Hallermayer, Hans-Peter Josel, Christian Klein, Josef Maier, Alexander Makower, Helmut Oberpriller, and Josef Ritter "Biosensor technology for the detection of illegal drugs II: antibody development and detection techniques", Proc. SPIE 2276, Cargo Inspection Technologies, (6 October 1994); https://doi.org/10.1117/12.189163

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