Modeling of 3D imaging in Brightfield microscope systems

Colin J. R. Sheppard; Min Gu

doi:10.1117/12.188053

30 September 1994 Modeling of 3D imaging in Brightfield microscope systems

Colin J. R. Sheppard, Min Gu

Proceedings Volume 2302, Image Reconstruction and Restoration; (1994) https://doi.org/10.1117/12.188053
Event: SPIE's 1994 International Symposium on Optics, Imaging, and Instrumentation, 1994, San Diego, CA, United States

Abstract

The way in which a reflection or transmission microscope image depends on the variations in complex refractive index in the object is considered. Reflection objects including rough surfaces and stratified media are discussed, and a generalized form for the scattering potential for reflection imaging in the Born approximation proposed. Reconstruction of the refractive index variation in a stratified medium is considered. Transmission imaging formation based on the Born and Rytov approximations is discussed. These properties are important for interpretation of the resulting images, and also as a basis for digital restoration methods.

Citation Download Citation

Colin J. R. Sheppard and Min Gu "Modeling of 3D imaging in Brightfield microscope systems", Proc. SPIE 2302, Image Reconstruction and Restoration, (30 September 1994); https://doi.org/10.1117/12.188053

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