Pharmacokinetics of meso-(tetrahydroxyphenyl)chlorin (m-THPC) studied by fluorescence spectroscopy on early cancer of the cheek pouch mucosa of Golden Syrian hamsters

Thomas M. Glanzmann; Jean-Francois Theumann; Daniel Braichotte; Martin Forrer; Georges A. Wagnieres; Hubert van den Bergh; Snezana Andrejevic-Blant; Jean-Francois Savary; Philippe Monnier

doi:10.1117/12.198706

4 January 1995 Pharmacokinetics of meso-(tetrahydroxyphenyl)chlorin (m-THPC) studied by fluorescence spectroscopy on early cancer of the cheek pouch mucosa of Golden Syrian hamsters

Thomas M. Glanzmann, Jean-Francois Theumann, Daniel Braichotte, Martin Forrer, Georges A. Wagnieres, Hubert van den Bergh, Snezana Andrejevic-Blant, Jean-Francois Savary, Philippe Monnier

Author Affiliations +

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198706
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Golden Syrian hamsters are evaluated as an animal model for phototherapy of early squamous cell carcinomas of the mucosa of the upper aerodigestive tract, the esophagus and the tracheobronchial tree. Carcinomas of this type are induced on the hamster cheek pouch mucosa by the application of the carcinogen 7,12 DMBA. For phototherapeutic experiments on the animals we utilized meso- (tetrahydoxyphenyl)chlorin (mTHPC). The same drug is currently in phase I, II clinical trials for ENT patients with superficial squamous cell carcinomas. By means of light induced fluorescence (LIF) we measured in vivo the kinetics of the uptake and removal of mTHPC in the normal and tumoral cheek mucosa and in the skin. The photodynamic therapy (PDT) reaction of the tissue after excitation of the photosensitizer by laser light at 652 nm was studied. Both pharmacokinetics and PDT efficacy are compared between animal model and clinical results with special emphasis on selectivity between normal and tumoral mucosa. These first experiments show that this tumor model in the hamster cheek pouch seems to be suitable for tests of a number of PDT variables of new photosensitizers preceding their clinical application as well as for optimization of the multiple parameters of clinical phototherapy.

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Thomas M. Glanzmann, Jean-Francois Theumann, Daniel Braichotte, Martin Forrer, Georges A. Wagnieres, Hubert van den Bergh, Snezana Andrejevic-Blant, Jean-Francois Savary, and Philippe Monnier "Pharmacokinetics of meso-(tetrahydroxyphenyl)chlorin (m-THPC) studied by fluorescence spectroscopy on early cancer of the cheek pouch mucosa of Golden Syrian hamsters", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198706

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