Spectroscopy and microscopy studies of skin tissue autofluorescence emission

Haishan Zeng; Calum E. MacAulay; David I. McLean M.D.; Branko Palcic

doi:10.1117/12.198720

4 January 1995 Spectroscopy and microscopy studies of skin tissue autofluorescence emission

Haishan Zeng, Calum E. MacAulay, David I. McLean M.D., Branko Palcic

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198720
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

The spectroscopic and microscopic characteristics of skin tissue autofluorescence emission were studied using a combined fluorescence and reflectance spectroanalyzer and a fiber optic microspectrophotometer. The autofluorescence spectra of in vivo human skin were measured over a wide excitation wavelength range (350 nm - 470 nm). The excitation emission matrices (EEMs) of in vivo skin were obtained. An excitation-emission maximum pair (380 nm, 470 nm) was identified. It was revealed that the most probable energy of skin autofluorescence emission photons increases linearly with increasing excitation photon energy. It was demonstrated that the diffuse reflectance R can be used as a first order approximation of the fluorescence distortion factor f to correct the measured in vivo autofluorescence spectra for the effect of tissue reabsorption and scattering. The microscopic in vitro autofluorescence properties of excised skin tissue sections were examined using 442 nm He-Cd laser light excitation as an example. It was found that the fluorophore distribution inside the skin tissue is not uniform and the shapes of the autofluorescence spectra of different anatomical skin layers vary. Using the obtained in vitro microscopic data, published skin optical parameters, and Monte Carlo simulation, the in vivo fluorescence emission spectrum was reconstructed and compared with the measurement results.

Citation Download Citation

Haishan Zeng, Calum E. MacAulay, David I. McLean M.D., and Branko Palcic "Spectroscopy and microscopy studies of skin tissue autofluorescence emission", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198720

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