Nonlinear optical method for the investigation of spectral properties of biomolecular complexes: second harmonic generation in ordered structures of bacteriorhodopsin

Oleg A. Aktsipetrov; Andrew A. Fedyanin; Tatyana V. Murzina; G. P. Borisevich; A. A. Kononenko

doi:10.1117/12.200878

1 February 1995 Nonlinear optical method for the investigation of spectral properties of biomolecular complexes: second harmonic generation in ordered structures of bacteriorhodopsin

Oleg A. Aktsipetrov, Andrew A. Fedyanin, Tatyana V. Murzina, G. P. Borisevich, A. A. Kononenko

Author Affiliations +

Proceedings Volume 2329, Optical and Imaging Techniques in Biomedicine; (1995) https://doi.org/10.1117/12.200878
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

For the first time the method of the second harmonic generation was used to study the photo- and electrically induced nonlinear optical transformations in thin oriented films of purple membranes (PM). Variations of the film nonlinear susceptibility were investigated as the bacteriorhodopsin (bR) molecule underwent the cycle of photoinduced transformations for both dry electrically oriented films and bR molecules embedded into poly(vinyl alcohol) matrix. The electrically induced changes of the nonlinear optical properties were studied for the electrostatic field strength up to the values 4 (DOT) 10⁴ V/cm. Nonlinear susceptibilities of oriented and nonoriented dried PM films are compared.

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Oleg A. Aktsipetrov, Andrew A. Fedyanin, Tatyana V. Murzina, G. P. Borisevich, and A. A. Kononenko "Nonlinear optical method for the investigation of spectral properties of biomolecular complexes: second harmonic generation in ordered structures of bacteriorhodopsin", Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); https://doi.org/10.1117/12.200878

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