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10 February 1995 Sensing pads for hybrid and monolithic integrated optical immunosensors
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Proceedings Volume 2331, Medical Sensors II and Fiber Optic Sensors; (1995) https://doi.org/10.1117/12.201232
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France
Abstract
Results of theoretical and experimental investigations are reported on the feasibility and the performance of a highly sensitive and stable `standardized' sensing pad suitable for integration into a great variety of miniature hybrid and monolithic integrated optical sensors for biochemical and clinical analysis. The basic type of sensing pad structure consists of sensing layers immobilized at the surface of waveguiding films with a high refractive index deposited on low index substrates. As a simple biochemical model system for the sensing pad development, the binding of immunoglobulin (IgG) to immobilized protein A was chosen. Results obtained for TiO2 waveguides at wavelengths of 633 nm and 785 nm show that these sensing pads feature very high sensitivities and an excellent stability, and that infrared laser diodes are adequate light sources. Resolutions and stabilities in the order of 1/4000 of a monolayer have been demonstrated by performing experiments for such chips in an input grating coupler configuration. Based on these results, surface coverage resolutions in the < 100 fg/mm2 range have been estimated if such sensing pads were used in integrated optical interferometers.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Rino E. Kunz, Gert L. Duveneck, and Markus Ehrat "Sensing pads for hybrid and monolithic integrated optical immunosensors", Proc. SPIE 2331, Medical Sensors II and Fiber Optic Sensors, (10 February 1995); https://doi.org/10.1117/12.201232
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