Thin-film microelectric arrays for amperometric enzyme biosensors with electrochemically synthesized glucose oxidase-polyaniline membrane

Sergei V. Dzyadevich; Victor K. Rossokhaty; Nataly Shram; Alexander A. Shul'ga; Alexey P. Soldatkin; Vitaly I. Strikha

doi:10.1117/12.190723

26 October 1994 Thin-film microelectric arrays for amperometric enzyme biosensors with electrochemically synthesized glucose oxidase-polyaniline membrane

Sergei V. Dzyadevich, Victor K. Rossokhaty, Nataly Shram, Alexander A. Shul'ga, Alexey P. Soldatkin, Vitaly I. Strikha

Author Affiliations +

Proceedings Volume 2364, Second International Conference on Thin Film Physics and Applications; (1994) https://doi.org/10.1117/12.190723
Event: Thin Film Physics and Applications: Second International Conference, 1994, Shanghai, China

Abstract

An amperometric glucose biosensor was fabricated by the electrochemical polymerization of aniline onto a gold electrodes in presence of glucose oxidase in phosphate buffer solution, pH 7.0. Aniline is easily polymerized forming a thin film, which adheres tightly on the electrodes surface. During the electropolymerization process the enzyme was entrapped into the polyaniline film being able to catalyze the hydrolysis of glucose. The experiments were performed to determine the optimal condition for polyaniline-glucose oxidase film preparation. Glucose can be determined by the biosensor in the concentration range 10^-4 M to 2 X 10^-2 M. The linearity of the biosensor response was observed from 2 X 10^-4 M to 6 X 10^-3 M glucose, which demonstrated that the internal diffusion of substrates and products of reaction through the polyaniline layer to the electrodes surface was the main limiting factor controlling the response value. The method of electropolymerization was found to have several advantage in comparison with other approaches especially for further mass manufacturing of the biosensors.

Citation Download Citation

Sergei V. Dzyadevich, Victor K. Rossokhaty, Nataly Shram, Alexander A. Shul'ga, Alexey P. Soldatkin, and Vitaly I. Strikha "Thin-film microelectric arrays for amperometric enzyme biosensors with electrochemically synthesized glucose oxidase-polyaniline membrane", Proc. SPIE 2364, Second International Conference on Thin Film Physics and Applications, (26 October 1994); https://doi.org/10.1117/12.190723

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