Cellular mechanisms of lipophilic cationic drug distribution: a confocal laser microspectrofluorometric assessment

F. Sureau; J. M. Kuhnel; M. A. Schwaller; Pierre-Yves Turpin

doi:10.1117/12.197407

2 January 1995 Cellular mechanisms of lipophilic cationic drug distribution: a confocal laser microspectrofluorometric assessment

F. Sureau, J. M. Kuhnel, M. A. Schwaller, Pierre-Yves Turpin

Author Affiliations +

Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197407
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

Ellipticine has been taken as a model for antitumoral compounds having weak base properties (7 < pK < 8.5). The protonation state and intracellular distribution of the drug were investigated in single living cells by confocal laser microspectrofluorimetry. These processes appear to be mainly mediated by transmembrane electric potentials and subcellular local pHs, as well as by the modification of the protonation equilibrium of the drug when bound to various biological macromolecules.

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F. Sureau, J. M. Kuhnel, M. A. Schwaller, and Pierre-Yves Turpin "Cellular mechanisms of lipophilic cationic drug distribution: a confocal laser microspectrofluorometric assessment", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197407

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