Experimental determination of lifetimes of MC 540 in organic solvents and model membrane systems

Dusan Chorvat; Libusa Sikurova

doi:10.1117/12.197411

2 January 1995 Experimental determination of lifetimes of MC 540 in organic solvents and model membrane systems

Dusan Chorvat, Libusa Sikurova

Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197411
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

The fluorescence lifetimes of merocyanine 540 (MC 540) in ethanol, methanol, and suspensions of DMPC and DPPC liposomes were studied using a laser-based time-correlated single photon counting system. Time-resolved fluorescence of MC 540 in methanol and ethanol can be analyzed with a single-exponential model, fluorescence decays of MC 540 in lecithin suspensions show biexponential behavior. We also present basic information about time-resolved fluorescence analysis software, designed and used by the authors as an integrated data station.

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Dusan Chorvat and Libusa Sikurova "Experimental determination of lifetimes of MC 540 in organic solvents and model membrane systems", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197411

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