Photothermal technique in cell microscopy studies

Dmitry Lapotko; Igor Chebot'ko; Georgy Kutchinsky; Sergey Cherenkevitch

doi:10.1117/12.197483

2 January 1995 Photothermal technique in cell microscopy studies

Dmitry Lapotko, Igor Chebot'ko, Georgy Kutchinsky, Sergey Cherenkevitch

Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197483
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

Photothermal (PT) method is applied for a cell imaging and quantitative studies. The techniques for cell monitoring, imaging and cell viability test are developed. The method and experimental set up for optical and PT-image acquisition and analysis is described. Dual- pulsed laser set up combined with phase contrast illumination of a sample provides visualization of temperature field or absorption structure of a sample with spatial resolution 0.5 micrometers . The experimental optics, hardware and software are designed using the modular principle, so the whole set up can be adjusted for various experiments: PT-response monitoring or photothermal spectroscopy studies. Sensitivity of PT-method provides the imaging of the structural elements of live (non-stained) white blood cells. The results of experiments with normal and subnormal blood cells (red blood cells, lymphocytes, neutrophyles and lymphoblasts) are reported. Obtained PT-images are different from optical analogs and deliver additional information about cell structure. The quantitative analysis of images was used for cell population comparative diagnostic. The viability test for red blood cell differentiation is described. During the study of neutrophyles in norma and sarcoidosis disease the differences in PT-images of cells were found.

Citation Download Citation

Dmitry Lapotko, Igor Chebot'ko, Georgy Kutchinsky, and Sergey Cherenkevitch "Photothermal technique in cell microscopy studies", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197483

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