In vivo monitoring of photosensitizer fluorescence during photodynamic therapy

Mark R. Stringer; Dominic J. Robinson; Emma J. Hudson; Michael A. Smith

doi:10.1117/12.203433

1 March 1995 In vivo monitoring of photosensitizer fluorescence during photodynamic therapy

Mark R. Stringer, Dominic J. Robinson, Emma J. Hudson, Michael A. Smith

Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203433
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States

Abstract

A method is presented of monitoring the low level fluorescence emitted by the photosensitizing agent protoporphyrin IX during superficial photodynamic therapy of skin carcinomas, using 630 nm illumination. A fiber optic probe samples the light field which is filtered and recorded by an optical spectrum analyzer. The technique is minimally invasive and can proceed concurrently with light dosimetry measurements. This paper presents in vitro data that define the sensitivity and selectivity of the technique, along with preliminary in vivo measurements. These indicate that it is the rate of phototransformation of the photosensitizer, rather than the total light dose, that determines the optimum treatment duration. Clinically effective treatment therefore depends upon achieving a threshold concentration of drug throughout the volume of the lesion. In this way the effect of phototransformation does not inactivate the drug before complete tumor necrosis occurs.

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Mark R. Stringer, Dominic J. Robinson, Emma J. Hudson, and Michael A. Smith "In vivo monitoring of photosensitizer fluorescence during photodynamic therapy", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203433

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