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1 March 1995 Tunable laser-based illumination system for cells in monolayer
Janet E. Cruse-Sawyer, Mark R. Stringer
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Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203410
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States
Abstract
A system of testing of potential photosensitizing agents for PDT by the illumination of cells in monolayer is described. The system allows tunability of the excitation wavelength, uniform illumination over a 35 mm cell dish, variation of the irradiance and monitoring of the power delivered throughout the duration of illumination. Knowledge of these parameters allows rapid processing, precise light dosimetry, limitation of possible heating effects as well as optimization of the excitation efficiency. The system is applied to the illumination of an endothelial hybridoma cell line using 680 nm and 692 nm light in the presence of phthalocyanine sensitizers.
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Janet E. Cruse-Sawyer and Mark R. Stringer "Tunable laser-based illumination system for cells in monolayer", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203410
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KEYWORDS
Laser systems engineering
Tunable lasers
Photodynamic therapy
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