Energy transfer of nucleic acid products

Paul M. Jung; Hsiang-Yun Hu; Omar S. Khalil

doi:10.1117/12.206013

3 April 1995 Energy transfer of nucleic acid products

Paul M. Jung, Hsiang-Yun Hu, Omar S. Khalil

Proceedings Volume 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics; (1995) https://doi.org/10.1117/12.206013
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Fluorescence energy transfer was investigated as a homogeneous detection method for the gapped ligase chain reaction (G-LCR). Oligonucleotides of a Chlamydia trachomatic G-LCR probe set were labeled with fluorescein as the donor and Texas Red as the acceptor fluorophore. Amplification and detection of 10 molecules of synthetic target was demonstrated in spiked urine samples.

Citation Download Citation

Paul M. Jung, Hsiang-Yun Hu, and Omar S. Khalil "Energy transfer of nucleic acid products", Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); https://doi.org/10.1117/12.206013

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