Ultrahigh-speed DNA sequencing using capillary array electrophoresis chips

Adam T. Woolley; Richard A. Mathies

doi:10.1117/12.206006

3 April 1995 Ultrahigh-speed DNA sequencing using capillary array electrophoresis chips

Adam T. Woolley, Richard A. Mathies

Proceedings Volume 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics; (1995) https://doi.org/10.1117/12.206006
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Capillary electrophoresis arrays have been fabricated on planar glass substrates using photolithographic masking and chemical etching. The photolithographically defined channel patterns were first etched in a glass substrate, and then capillaries were formed by thermally bonding the etched substrate to a second glass slide. High-speed separations of restriction fragment digests were performed on these chips in under 1 minute. Multiple separations in the same channel demonstrated excellent reproducibility. Rapid genetic typing of short tandem repeats on the HUMTHO1 locus have also been performed. Finally, separations with single- base resolution have been successful, indicating that these microfabricated devices will also be useful for high-speed DNA sequencing.

Citation Download Citation

Adam T. Woolley and Richard A. Mathies "Ultrahigh-speed DNA sequencing using capillary array electrophoresis chips", Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); https://doi.org/10.1117/12.206006

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