Effects of cryogenic grinding on soft tissue optical properties

Eric K. Chan; Thomas Menovsky; Ashley J. Welch

doi:10.1117/12.206814

12 April 1995 Effects of cryogenic grinding on soft tissue optical properties

Eric K. Chan, Thomas Menovsky, Ashley J. Welch

Proceedings Volume 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II; (1995) https://doi.org/10.1117/12.206814
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Optical properties of frozen-ground and intact soft tissues obtained from spectrophotometer measurements of reflectance and transmittance were compared. The tissues used in these experiments were calf aorta, rat jejunum, and rabbit sciatic nerve. Each tissue specimen was frozen in liquid nitrogen and then ground with pestle and mortar into a fine powder. A tissue paste formed once the powder returned to room temperature. The tissue paste was then sandwiched between glass slides for spectrophotometric measurements. For comparison, the optical properties of the intact tissues were also measured. Total transmission and diffuse reflection were obtained on a Varian Cary 5 Spectrophotometer (400 nm - 850 nm). Absorption and reduced scattering coefficients of the tissues were determined with the Inverse Adding Doubling Method. Our results suggested that optical properties of soft tissue can be estimated from that of the ground tissue in the visible range.

Citation Download Citation

Eric K. Chan, Thomas Menovsky, and Ashley J. Welch "Effects of cryogenic grinding on soft tissue optical properties", Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); https://doi.org/10.1117/12.206814

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