Fiber optic monitoring system for mouse embryo evaluation

Harumi Itoh; Tsunenori Arai; Makoto Kikuchi

doi:10.1117/12.208507

8 May 1995 Fiber optic monitoring system for mouse embryo evaluation

Harumi Itoh, Tsunenori Arai, Makoto Kikuchi

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208507
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

We constructed a fiber-optic long-term monitoring system for mouse embryo, which developed a new quantitative and non-invasive evaluation method for human embryo. We monitored the changes of light scattering from mouse embryo which might relate to the state of blastomere and change in intercellular microstructures. We employed a couple of optical fibers to measure the scattering. These fibers were arranged in a light angle to the optical axis of a microscope to obtain adaptability for various measurements. A He-Ne laser with 632.8 nm in wavelength was used as a light source. The emission fiber (56 micrometers (Phi) ; core) and sensing fiber (400 micrometers (Phi) ; core, NA equals 0.43) were used. We observed the light scattering changes from the embryo, which were over 200 times higher than the noise level with 30 s in period. This result might be attributed to the structural and/or functional changes in the mouse embryo.

Citation Download Citation

Harumi Itoh, Tsunenori Arai, and Makoto Kikuchi "Fiber optic monitoring system for mouse embryo evaluation", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208507

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available