Integrated optic immunoassay for virus detection

Anthony A. Boiarski; James R. Busch; Larry S. Miller; A. W. Zulich; James Burans

doi:10.1117/12.208519

8 May 1995 Integrated optic immunoassay for virus detection

Anthony A. Boiarski, James R. Busch, Larry S. Miller, A. W. Zulich, James Burans

Author Affiliations +

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208519
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

An integrated optic refractometer device was developed to perform a rapid one-step, label-free immunoassay. The device measures refractive index changes at the surface of a planar waveguide using interferometry. Antibodies were applied to the waveguide surface to provide a bioselective coating for detecting and quantifying a specific antigen of interest. The detection limit of this biosensor was determined for adenovirus as a model for other viral analytes of military, medical, and environmental interest. As binding of the antigen occurred on the sensor surface, a time-dependent phase shift of the helium-neon laser light beam was detected and was measured over a 10-minute time period. Adenovirus was detected at levels of 250 - 2500 viral particles/ml. This detection limit was obtained for a mono-layer of antibody attached to the sensor. Use of a high-density, multi-layer antibody coating approach resulted in improved detection limits for bacteria and protein analytes of general interest.

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Anthony A. Boiarski, James R. Busch, Larry S. Miller, A. W. Zulich, and James Burans "Integrated optic immunoassay for virus detection", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208519

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