Laser-induced fluorescence endoscopic imaging for detection of colonic dysplasia

Thomas D. Wang; Yang Wang; Jacques Van Dam; James M. Crawford; Elizabeth Preisinger; Michael S. Feld

doi:10.1117/12.205987

30 March 1995 Laser-induced fluorescence endoscopic imaging for detection of colonic dysplasia

Thomas D. Wang, Yang Wang, Jacques Van Dam, James M. Crawford, Elizabeth Preisinger, Michael S. Feld

Proceedings Volume 2390, Optical Biophysics; (1995) https://doi.org/10.1117/12.205987
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

We are studying the use of the laser-induced fluorescence (LIF) endoscopic images of colonic mucosa for detection of pre-malignant lesions. LIF images were collected through a fiber optic colonoscope, and adenomatous polyps were used as a model of dysplasia. A total of 12 tissue samples containing 29 adenomas, obtained from colectomy specimens from 3 familial adenomatous polyposis patients, were studied. Regions of colonic mucosa were illuminated by a quartz optical fiber with near-UV light from an argon-ion laser. Autofluorescence between 400 and 700 nm was detected by means of an intensified CID camera. In the LIF images, adenomatous polyps appeared lower in intensity than normal mucosa by about a factor of 2. The LIF images were processed by dividing the raw image by a spatially averaged one to correct for differences in the distance to the tissue and in the light collection efficiency of the optics. Relative intensity thresholds were set at values varying between 55% and 90% compared to the spatial average to determine likely areas of disease. The results were compared to histology taken at 2 mm intervals along several transverse cross-sections of the specimens. At a threshold of 75%, 26 true positives, 256 true negatives, 22 false positives, and 3 false negatives were identified, resulting in a sensitivity, specificity, positive predictive value and percentage of correct determinations of 90%, 92%, 54%, and 92%, respectively. These values are comparable to results of independent diagnoses by two pathologists, demonstrating the potential of LIF to guide biopsy.

Citation Download Citation

Thomas D. Wang, Yang Wang, Jacques Van Dam, James M. Crawford, Elizabeth Preisinger, and Michael S. Feld "Laser-induced fluorescence endoscopic imaging for detection of colonic dysplasia", Proc. SPIE 2390, Optical Biophysics, (30 March 1995); https://doi.org/10.1117/12.205987

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