Tissue dependent biosynthesis and pharmacokinetics of Protopophyrin IX following intravenous injection of <delta>-aminolevulinic acid

Avigdor M. Ronn; Lennart A. Lofgren M.D.

doi:10.1117/12.208177

1 May 1995 Tissue-dependent biosynthesis and pharmacokinetics of Protopophyrin IX following intravenous injection of δ-aminolevulinic acid

Avigdor M. Ronn, Lennart A. Lofgren M.D.

Author Affiliations +

Proceedings Volume 2392, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV; (1995) https://doi.org/10.1117/12.208177
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Two significant questions arise when a drug for photodynamic therapy is introduced to the preclinical arena: (1) pharmacokinetics for the uptake and elimination and (2) relative retention by various tissues. Theses were addressed by following the pharmacokinetics in 21 rabbits with 130 tumors at different drug doses and at time intervals ranging from minutes to 24 hours. Kinetics were studied spectrofluorometrically by following extracting Protoporphyrin IX (PPIX), a metabolite of delta- aminolevulinic acid (ALA). Samples were compared to a standard, and are thus quantitative. The rise time in plasma was rapid, and reached maximum one hour post injection with drug doses of 50, 100, and 200 mg/kg. The single exponential fall time displayed a half life of fifty minutes, resulting in a complete elimination of PPIX in 24 hours. Uptake and retention in multiple tissues were measured 3 hours post injection of 100 mg/g ALA in 3 animals, with skin, tumor, and plasma measured in all 21 animals. Organs with high vascularity displayed higher values of PPIX. Results of this study indicate that ALA utilized as a systemically administered sensitizer maybe of significant value in PDT of selected tumor types.

Citation Download Citation

Avigdor M. Ronn and Lennart A. Lofgren M.D. "Tissue-dependent biosynthesis and pharmacokinetics of Protopophyrin IX following intravenous injection of δ-aminolevulinic acid", Proc. SPIE 2392, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV, (1 May 1995); https://doi.org/10.1117/12.208177

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available