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23 March 1995 Polarization contrast in scanning microscopy
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Proceedings Volume 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II; (1995) https://doi.org/10.1117/12.205323
Event: IS&T/SPIE's Symposium on Electronic Imaging: Science and Technology, 1995, San Jose, CA, United States
Abstract
We discuss the differences between polarization contrast imaging in conventional and confocal systems and show that because of the fundamentally different imaging properties of the two systems, the extinction coefficient is non-zero in a conventional system even with perfect polars. We then discuss the axial response of high numerical aperture systems and show that a polarization effect leads to an asymmetric response when the objective lens numerical aperture is greater than the refractive index of the specimen.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Tony Wilson and Rimas Juskaitis "Polarization contrast in scanning microscopy", Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); https://doi.org/10.1117/12.205323
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