Absorption and emission spectroscopy in the analysis of laser radiation and fast neutron action on chromatin

Liliana Radu; Ion N. Mihailescu; B. Constantinescu; O. Gostian

doi:10.1117/12.203584

8 March 1995 Absorption and emission spectroscopy in the analysis of laser radiation and fast neutron action on chromatin

Liliana Radu, Ion N. Mihailescu, B. Constantinescu, O. Gostian

Author Affiliations +

Proceedings Volume 2461, ROMOPTO '94: Fourth Conference in Optics; (1995) https://doi.org/10.1117/12.203584
Event: ROMOPTO '94: 4th Conference on Optics, 1994, Bucharest, Romania

Abstract

The actions of a 248 mm excimer laser radiation (0.5 - 3 MJ/m2) and of fast neutrons (d(13 MeV) + Be thick target, 5 - 100 Gy) on chromatin (the complex of DNA and proteins of eukaryotic cells) were analyzed. The parameters estimated were: the thermal transition, the fluorescence of chromatin-ethidium bromide complexes, the chromatin intrinsic fluorescence and the energy transfer efficiency between specific fluorescent ligands coupled at chromatin. The mechanism of UV excimer laser action is similar to the fast neutrons action on chromatin. The complex action includes the production of chromatin DNA strand breaks, the protein structure lesion and the chromatin conformation modification.

Citation Download Citation

Liliana Radu, Ion N. Mihailescu, B. Constantinescu, and O. Gostian "Absorption and emission spectroscopy in the analysis of laser radiation and fast neutron action on chromatin", Proc. SPIE 2461, ROMOPTO '94: Fourth Conference in Optics, (8 March 1995); https://doi.org/10.1117/12.203584

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