Amperometric biosensors for the determination of heavy metals

Dario Compagnone; Giuseppe Palleschi; Giuseppe Varallo; PierLuigi Imperiali

doi:10.1117/12.224096

9 October 1995 Amperometric biosensors for the determination of heavy metals

Dario Compagnone, Giuseppe Palleschi, Giuseppe Varallo, PierLuigi Imperiali

Proceedings Volume 2504, Environmental Monitoring and Hazardous Waste Site Remediation; (1995) https://doi.org/10.1117/12.224096
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany

Abstract

A bioelectrochemical method for the determination of heavy metal ions has been developed. This method is based on the inhibition effect of metal ions on the enzymatic activity of oxidase enzymes. The enzymatic activity was determined with an amperometric hydrogen peroxide probe. The inhibition effect on enzymes in solution and covalently immobilized on polymeric supports has been evaluated. Hg(II) was the metal ion that inhibited almost all the enzymes, particularly glycerol-3-P oxidase. Hg(II) was detected in the 0.05/0.5 ppm range with the enzyme in solution. Calibration curves for Hg(II) were also obtained with the other oxidase enzymes in the 0.5/10 ppm range. The other metal ions tested inhibited the enzymes more specifically. The metal ion/enzyme systems which gave the best inhibition were Se(IV)/glutathione oxidase, Ni(II)/sarcosine oxidase, V(V)/glutathione oxidase, Cu(II)/alcohol oxidase from Pichia Pastoris and Cd(II)/D-aminoacid oxidase. All these metal ions were detected in the 0.1/10 ppm range using the enzymes in solution or covalently immobilized.

Citation Download Citation

Dario Compagnone, Giuseppe Palleschi, Giuseppe Varallo, and PierLuigi Imperiali "Amperometric biosensors for the determination of heavy metals", Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); https://doi.org/10.1117/12.224096

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