Atomic force microscopy and molecular modeling of protein bound to calcite surfaces

C. Steven Sikes; Andre Wierzbicki

doi:10.1117/12.221505

25 September 1995 Atomic force microscopy and molecular modeling of protein bound to calcite surfaces

C. Steven Sikes, Andre Wierzbicki

Proceedings Volume 2547, Laser Techniques for Surface Science II; (1995) https://doi.org/10.1117/12.221505
Event: SPIE's 1995 International Symposium on Optical Science, Engineering, and Instrumentation, 1995, San Diego, CA, United States

Abstract

Proteins that regulate inorganic crystal formation in organisms are in some instances irreversibly bound to the mineral phase. They are clearly resolved by atomic force microscopy, easily withstanding the forces of the AFM probe in contact mode. This is demonstrated in AFM images of proteins attached to fragments of calcite oyster shell. The proteins may take specific positions on the crystal surface, in effect becoming part of the surficial lattice, stereochemically matching the functional groups of the protein with lattice positions of the constituent ions of the crystal. Requirements for adsorbate binding that is strong enough for AFM viewing in contact mode are discussed, including comparisons of ionically driven binding of oyster shell protein to calcite and hydrogen-bonded antifreeze proteins to ice.

Citation Download Citation

C. Steven Sikes and Andre Wierzbicki "Atomic force microscopy and molecular modeling of protein bound to calcite surfaces", Proc. SPIE 2547, Laser Techniques for Surface Science II, (25 September 1995); https://doi.org/10.1117/12.221505

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