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1 September 1995 Confocal scanning microscopy under ultrashort pulse illumination
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Abstract
We, in this paper, investigate resolution in various confocal imaging methods based on the use of an ultrashort pulsed laser beam. For bright-field confocal microscopy, time-resolved and time averaged imaging methods are considered, while resolution in two-photon and single- photon confocal fluorescence microscopy is examined. It is shown that the time-resolved approach can give a 3D image of a thick object with axial resolution approximately 30% higher than the limiting value under CW laser illumination of the same wavelength as the pulsed beam, while the transverse resolution is slightly improved.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Min Gu, T. Tannous, and Colin J. R. Sheppard "Confocal scanning microscopy under ultrashort pulse illumination", Proc. SPIE 2549, Ultrahigh- and High-Speed Photography, Videography, and Photonics '95, (1 September 1995); https://doi.org/10.1117/12.218311
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