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15 January 1996 Two-photon excited cellular autofluorescence induced by cw and femtosecond NIR microradiation
Karsten Koenig, Peter T. C. So, William W. Mantulin, Enrico Gratton, Tatiana B. Krasieva, Michael W. Berns, Bruce J. Tromberg
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Proceedings Volume 2628, Optical and Imaging Techniques for Biomonitoring; (1996) https://doi.org/10.1117/12.229988
Event: BiOS Europe '95, 1995, Barcelona, Spain
Abstract
We report the first two-photon excited autofluorescence measurements in single cells induced by continuous wave NIR laser microbeams. In particular we demonstrate NIR-excited NAD(P)H fluorescence of motile spermatozoa in a single-beam gradient force optical trap ('optical tweezers'). In addition, two-photon NIR excited autofluorescence imaging and 'tau- mapping' of CHO cells was performed with laser scanning microradiation of a tunable femtosecond-Ti:sapphire laser. Cellular response on autofluorescence to two-photon absorption of NIR radiation was compared with exposure to UVA light (one-photon absorption).
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Karsten Koenig, Peter T. C. So, William W. Mantulin, Enrico Gratton, Tatiana B. Krasieva, Michael W. Berns, and Bruce J. Tromberg "Two-photon excited cellular autofluorescence induced by cw and femtosecond NIR microradiation", Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996); https://doi.org/10.1117/12.229988
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KEYWORDS
Luminescence
Near infrared
Continuous wave operation
Laser scanners
Absorption
Femtosecond phenomena
Microscopes
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