UVA-induced oxidative stress in single cells probed by autofluorescence modifications, cloning assay, and comet assay

Karsten Koenig; Tatiana B. Krasieva; Eckhard Bauer; Ulrich Fiedler; Michael W. Berns; Bruce J. Tromberg; Karl-Otto Greulich

doi:10.1117/12.230020

15 January 1996 UVA-induced oxidative stress in single cells probed by autofluorescence modifications, cloning assay, and comet assay

Karsten Koenig, Tatiana B. Krasieva, Eckhard Bauer, Ulrich Fiedler, Michael W. Berns, Bruce J. Tromberg, Karl-Otto Greulich

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Proceedings Volume 2628, Optical and Imaging Techniques for Biomonitoring; (1996) https://doi.org/10.1117/12.230020
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Cell damage by low-power 365 nm radiation of a 50 W high-pressure mercury microscopy lamp was studied. UVA exposure to CHO cells resulted for radiant exposures greater than 10 kJ/m² in significant modifications of NADH-attributed autofluorescence and in inhibition of cell division. Single cell gel electrophoresis (comet assay) revealed UVA-induced single strand DNA breaks. According to these results, UVA excitation radiation in fluorescence microscopy may damage cells. This has to be considered in vital cell microscopy, e.g. in calcium measurements.

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Karsten Koenig, Tatiana B. Krasieva, Eckhard Bauer, Ulrich Fiedler, Michael W. Berns, Bruce J. Tromberg, and Karl-Otto Greulich "UVA-induced oxidative stress in single cells probed by autofluorescence modifications, cloning assay, and comet assay", Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996); https://doi.org/10.1117/12.230020

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