Spectral properties of coloidal gold and its conjugates with biospecific macromolecules

Nikolai G. Khlebtsov; Vladimir A. Bogatyrjov; Lev A. Dykman; Andrei G. Melnikov

doi:10.1117/12.229536

8 January 1996 Spectral properties of coloidal gold and its conjugates with biospecific macromolecules

Nikolai G. Khlebtsov, Vladimir A. Bogatyrjov, Lev A. Dykman, Andrei G. Melnikov

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229536
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

The current work was undertaken to develop optical methods for the controlled preparation of gold sols and conjugates with biospecific macromolecules. The extinction spectra of sols with the particle size and axial ratio polydispersity were calculated using Mie's theory, the T-matrix method, and various experimental sets of the bulk gold optical constants modified with regard to size-limiting effects. It was shown that the most generalized model including the size dependence of the imaginary part of the dielectric permeability and the size and shape polydispersity gave good agreement with the experimental extinction spectra for 5-, 10-, 24-, and 40-nm sols, as well as with the size dependence of the position and value of the extinction peak. Electron-microscopic and spectral measurements yielded calibration curves for efficient spectrophotometric control over the particle size and for estimation of the amount of restorer essential for the preparation of particles of a given size. The stabilizing concentrations and the extinction spectra of colloidal gold-ovomucoid conjugates were measured. A simplest two- layered spherical model was employed to elucidate basic changes in sol spectra after conjugation with specific biomacromolecules and to draw some conclusions about the conjugate shell structure.

Citation Download Citation

Nikolai G. Khlebtsov, Vladimir A. Bogatyrjov, Lev A. Dykman, and Andrei G. Melnikov "Spectral properties of coloidal gold and its conjugates with biospecific macromolecules", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229536

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