Room temperature phosphorescent sensing probe for detection of DNA

Marta Elena Diaz-Garcia; M. Roza-Fernandez

doi:10.1117/12.229181

3 January 1995 Room temperature phosphorescent sensing probe for detection of DNA

Marta Elena Diaz-Garcia, M. Roza-Fernandez

Proceedings Volume 2631, Medical and Fiber Optic Sensors and Delivery Systems; (1995) https://doi.org/10.1117/12.229181
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

The interaction between double-stranded DNA and a luminescent Pd-porphyrin complex has been studied by room temperature phosphorescence (RTP). Intercalation of the Pd complex into DNA in deoxygenated solution is accompanied by an observed enhanced RTP emission centered at 680 nm. Analysis of the RTP spectral data gave a value of 1.0 multiplied by 10⁶ M^-1 for the Pd complex-DNA association constant and the complex seemed to bind DNA at the GC rich environments. The spectroscopic features of this interaction and the analytical performance characteristics of the RTP method for DNA determination are evaluated. The implication of the use of the Pd-complex RTP probe in combination with time resolved luminescence measurements are discussed.

Citation Download Citation

Marta Elena Diaz-Garcia and M. Roza-Fernandez "Room temperature phosphorescent sensing probe for detection of DNA", Proc. SPIE 2631, Medical and Fiber Optic Sensors and Delivery Systems, (3 January 1995); https://doi.org/10.1117/12.229181

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