Determination of polarizability and surface concentration of biomolecules using surface plasmon resonance experiment

Yuri M. Shirshov; Vladimir I. Chegel; Yuri V. Subbota; E. P. Matsas; A. E. Rachcov; T. A. Sergeeva

doi:10.1117/12.226151

3 November 1995 Determination of polarizability and surface concentration of biomolecules using surface plasmon resonance experiment

Yuri M. Shirshov, Vladimir I. Chegel, Yuri V. Subbota, E. P. Matsas, A. E. Rachcov, T. A. Sergeeva

Author Affiliations +

Proceedings Volume 2648, International Conference on Optical Diagnostics of Materials and Devices for Opto-, Micro-, and Quantum Electronics; (1995) https://doi.org/10.1117/12.226151
Event: International Conference on Optical Diagnostics of Materials and Devices for Opto-, Micro-, and Quantum Electronics, 1995, Kiev, Ukraine

Abstract

Both surface molecules concentration Ns and polarizability Am can be determined from the surface plasmon experiment instead of effective layer thickness, which is usually determined. The new method of quantitative comparison of experimental and calculated surface plasmon resonance (SPR) curves is proposed. Mathematical minimization of curves difference is used in this case. It is shown, that it is possible to determine both Am and Nm from one measurement for accurate experiment and high quality optical system.

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Yuri M. Shirshov, Vladimir I. Chegel, Yuri V. Subbota, E. P. Matsas, A. E. Rachcov, and T. A. Sergeeva "Determination of polarizability and surface concentration of biomolecules using surface plasmon resonance experiment", Proc. SPIE 2648, International Conference on Optical Diagnostics of Materials and Devices for Opto-, Micro-, and Quantum Electronics, (3 November 1995); https://doi.org/10.1117/12.226151

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