Special investigation of somatotropin for different pH

Virginia Otero de Joshi; Herminia Gil; Silvia Contreras; Narahari V. Joshi; Luis Hernandez

doi:10.1117/12.238807

24 April 1996 Spectral investigation of somatotropin for different pH values

Virginia Otero de Joshi, Herminia Gil, Silvia Contreras, Narahari V. Joshi, Luis Hernandez

Proceedings Volume 2676, Biomedical Sensing, Imaging, and Tracking Technologies I; (1996) https://doi.org/10.1117/12.238807
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Spectral investigations of absorbance in deep ultra-violet region (from 200 nm to 350 nm) of (STM) was carried out for different pH values. On the high energy side the peak is located at 195 nm which is generally attributed to peptide bonds. This peak, as expected, does not show any shift with pH value (4.3 to 10.8). A rather broad peak is spread in the region from 200 nm to 240 nm which could be the superposition of the peaks corresponding to the absorption due to (alpha) helix and (beta) structure. This peak shows a red shift as pH value increases. The same hormone was glycated by a conventional method and the process was estimated with the absorption spectra. The results are discussed in the light of nonenzymatic glycation. It was found that glycation mucus somatotropin resistant towards the denaturation process.

Citation Download Citation

Virginia Otero de Joshi, Herminia Gil, Silvia Contreras, Narahari V. Joshi, and Luis Hernandez "Spectral investigation of somatotropin for different pH values", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); https://doi.org/10.1117/12.238807

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