Protein database searching with MSn spectra of polypeptides

Jimmy K. Eng; John R. Yates III

doi:10.1117/12.237626

1 April 1996 Protein database searching with MSⁿ spectra of polypeptides

Jimmy K. Eng, John R. Yates III

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237626
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Database searching with tandem mass spectra has been demonstrated as a powerful method to correlate peptides and proteins to gene sequences for identification. In this study preliminary data is shown for extending the cross-correlation database searching method to multi-stage mass spectra of polypeptides. This method may provide a means to directly identify large polypeptides or proteins without the necessity of proteolytic hydrolysis to produce peptides amenable to tandem mass spectrometry.

Citation Download Citation

Jimmy K. Eng and John R. Yates III "Protein database searching with MSⁿ spectra of polypeptides", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237626

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