Enhanced delivery of molecules into cells using laser-induced stress waves

Kunio Awazu; Steven L. Jacques

doi:10.1117/12.239570

7 May 1996 Enhanced delivery of molecules into cells using laser-induced stress waves

Kunio Awazu, Steven L. Jacques

Proceedings Volume 2681, Laser-Tissue Interaction VII; (1996) https://doi.org/10.1117/12.239570
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

This study investigated the ability of laser-induced stress waves to enhance delivery of molecules into cells. Our experiment involved irradiating a thin layer of gel containing India ink at the tip of a capillary tube containing cells. The laser pulses (10 ns, 532 nm) deposited energy in the gel layer and generated intense stress waves which propagated into the capillary tube and affected the cells. The gel surface of irradiation was open to the air therefore the boundary condition was a free surface. The cell medium contained fluorescein attached to dextran of various molecular weights. After repeated laser pulses, the cells were collected on a glass slide and observed by fluorescence microscopy. Irradiated cells were observed to have taken up dye relative to unirradiated controls. The results indicate the ability of stress waves to enhance the uptake of large molecular weight reagents by cells.

Citation Download Citation

Kunio Awazu and Steven L. Jacques "Enhanced delivery of molecules into cells using laser-induced stress waves", Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); https://doi.org/10.1117/12.239570

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