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25 March 1996 Integrated optic sensor for measuring aflatoxin-B1 in corn
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An integrated optic refractometer device was developed to perform a rapid one-step, homogeneous immunoassay. The device measures refractive index changes at the surface of a planar, singlemode, ion-exchange waveguide using difference interferometry. Anti-aflatoxin- B1 antibodies were attached to the waveguide surface to provide a bioselective coating for detecting and quantifying the aflatoxin-B1 antigen level in a sample. The detection limit of this small antigen must be determined using a competitive assay format. To determine feasibility of the competitive assay, we determined the biosensor response to a larger molecular weight competing antigen, namely HRP-labeled aflatoxin-B1. This labeled antigen will compete with unlabeled aflatoxin for binding sites on the sensor surface. Increased sample aflatoxin levels will result in a decreased time-dependent phase change of the helium-neon laser light beam. Phase change data were determined for various concentration levels of HRP-labeled aflatoxin- B1 antigen. The assay measurements were made over a 5-minute time period. Results indicated that a competitive assay is feasible. Future assay efforts should be able to demonstrate measurement of aflatoxin-B levels found in contaminated corn samples.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Anthony A. Boiarski, James R. Busch, R. S. Brody, Richard W. Ridgway, Wolf P. Altman, and C. Golden "Integrated optic sensor for measuring aflatoxin-B1 in corn", Proc. SPIE 2686, Integrated Optics and Microstructures III, (25 March 1996);

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