Detection of immunological reaction with the attenuated total reflection (ATR) technique

Ericka Rodriguez; Luis Efrain Regalado

doi:10.1117/12.231071

5 February 1996 Detection of immunological reaction with the attenuated total reflection (ATR) technique

Ericka Rodriguez, Luis Efrain Regalado

Proceedings Volume 2730, Second Iberoamerican Meeting on Optics; (1996) https://doi.org/10.1117/12.231071
Event: Second Iberoamerican Meeting on Optics, 1995, Guanajuato, Mexico

Abstract

The sensitivity of the attenuated total reflection (ATR) technique to surface modifications allows the study of biological or medical phenomena where solutions segregate to form a layer on the walls of the sample holder. We present an immunoassay study of IgG antigen reacting with specific antibodies in saline solution on an aluminum thin-film attached to a prism, forming the bottom of the container where the reaction takes place. During the reaction a layer is adsorbed on the aluminum film showing a resonant absorption peak in the reflected signal that shifts towards larger angles of incidence at every step of the reaction. These shifts are related to the optical properties and the thickness of the adsorbed layers and may be quantified. This work presents the experimental setup and qualitative results of this reaction.

Citation Download Citation

Ericka Rodriguez and Luis Efrain Regalado "Detection of immunological reaction with the attenuated total reflection (ATR) technique", Proc. SPIE 2730, Second Iberoamerican Meeting on Optics, (5 February 1996); https://doi.org/10.1117/12.231071

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available